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KMID : 1007520130220000205
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Food Science and Biotechnology
2013 Volume.22 No. 0 p.205 ~ p.212
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Statistical Optimization, Partial Purification, and Characterization of Coffee Pulp β-Glucosidase and Its Application in Ethanol Production
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Roopali N. Bhoite
P. N. Navya
Pushpa S. Murthy
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Abstract
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Extracellular β-glucosidase was produced using coffee pulp as a sole carbon source by Penicillium verrucosum by solid state fermentation and 897.36±59 U/ g enzyme activity was obtained. Increase in 2.21-fold of enzyme activity on optimizing the bioprocess parameters by response surface methodology based on central composite rotatable design is illustrated. Maximum production level of 1,991.17 U/g was obtained with optimum values of pH 4.2, moisture 66.8%, and fermentation duration of 56 h. The enzyme was partially purified and the enzyme activity was optimum at 50℃ temperature and at pH 6. The metal ions such as Mg2+, Zn2+, Ca2+, K+, detergents, and chelator such as EDTA were effective and further increased the β- glucosidase activity. On application of β-glucosidase for simultaneous saccharifiation and fermentation, 3.3% ethanol was obtained. Thus, this study provides insight on exploitation of P. verrucosum for synthesis of of β-glucosidase using coffee pulp which is available abundantly in coffee processing industries.
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KEYWORD
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coffee pulp, Penicillium verrucosum, response surface methodology (RSM), β-glucosidase activity, ethanol production
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